目錄
目錄………………………………………………………………………………………1
中文摘要…………………………………………………………………………………2
英文摘要…………………………………………………………………………………2
一．前言 …………………………………………………………………………………3
二．實驗儀器與試藥 ……………………………………………………………………4
2.1 儀器………………………………………………………………………………4
2.2 試藥………………………………………………………………………………5
2.3 試劑………………………………………………………………………………5
三.實驗操作流程圖 …………………………………………………………………… 6
四．黃獨乙素的提取 ……………………………………………………………………7
4.1 回流提取 ………………………………………………………………………7
4.2 萃取………………………………………………………………………………8
4.3 小結與討論………………………………………………………………………8
五.黃獨乙素的分離 ……………………………………………………………………8
5.1 色譜柱分離 ……………………………………………………………………8
5.2 薄層板的檢測……………………………………………………………………8
5.3 埃利希檢測………………………………………………………………………8
5.4 小結與討論 ……………………………………………………………………9
六.化學成分鑒別………………………………………………………………………9
6.1HPCL色譜條件 …………………………………………………………………9
6.2 HPCL檢測………………………………………………………………………10
6.3小結與討論 ……………………………………………………………………10
參考文獻…………………………………………………………………………………10
致謝………………………………………………………………………………………11
綜述………………………………………………………………………………………13
個人簡介…………………………………………………………………………………19


中文摘要

摘要：目的：提取與分離黃藥子的毒性成分黃獨乙素。方法：回流提取，用三氯甲烷萃取提取液，通過反復常壓硅膠柱色譜分離從黃藥子三氯甲烷萃取部位中共得該化合物，利用物化性質(zhì)和譜學手段鑒定這個化合物的結構：黃獨乙素。結果：目標成分經(jīng)重結晶得大量白色柱狀結晶（埃利希反應呈陽性）。結論：利用硅膠柱色譜和梯度洗脫能夠較好地從黃藥子中篩選、分離出的有效成分黃獨乙素。

關鍵字：黃藥子；提?。环蛛x；鑒定




英文摘要

Abstract: Objective: Extraction and Separation of toxic components Dioscorea bulbifera Dioscorea bulbifera B factor. Methods: The extraction and extraction with chloroform extract by repeated silica gel column chromatography atmospheric pressure bulbifera chloroform extraction from the Chinese Communists were part of the compound, using physical and chemical properties and spectroscopic identification of the compounds means: B prime bulbifera. Results: The target component was crystallized at large white columnar crystal (positive Ehrlich reaction). Conclusion: The use of silica gel column chromatography and gradient elution can be well screened from Dioscorea bulbifera, isolated the active ingredient bulbifera B factor.

Keywords: Dioscorea bulbifera; extraction; separation; identification